Microfluidic Methods for Molecular Biology

Posted on September 6, 2016 by Admin

Book Description

Authors: Lu, Chang, Verbridge, Scott S

May 15th, 2016 Springer

Summary

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry.
This book also:

Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Table of Contents

Microfluidic Platforms for Quantitative Biology Studies in Model Organisms
Microfluidic Methods in Single Cell Biology
Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics
Forensic Typing of Single Cells Using Droplet Microfluidics
Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA Targets in Single Cells
Microfluidic Multistage Integration for Analysis of Circulating Exosomes
Microfluidic Single-Cell Functional Proteomics
Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays
Single-Cell Phenotypic Screening in Inverse Metabolic Engineering
Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers
Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression
Wire-guided Droplet Manipulation for Molecular Biology
Mechanical and Electrical Principles for Separation of Rare Cells
Microfluidics for High-Throughput Cellular Isolation and Analysis in Biomedicine
Microfluidics for Cell Culture
Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations