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MUSC Advanced Flow Cytometry Training

March 28, 2017 - March 29, 2017

Educational Objectives – This two-day course will start by covering the fundamental areas critical for successful flow cytometry: Reagents, Hardware, Controls, Compensation, and Data Analysis. The second day will build upon these concepts with a focus on practical application of flow cytometry including: Polychromatic Panel Design, Statistics, Cell Cycle analysis, Apoptosis and Troubleshooting. At the end of the course, attendee’s will have firm understanding of the principles and how these apply to developing, executing, analyzing and troubleshooting flow cytometry experiments

Target Audience: Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color experiments). Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.

Day 1 – Principles of Flow Cytometry

09:00-09:30 Course Introduction and Activity 1 – Design the Experiment

09:30-10:15 Lecture 1 – Software (Reagents, Fluorescence and Fluorochromes)

10:15-10:30 Introduction to Spectral Viewers

10:30-10:45 Coffee Break and questions

10:45-11:30 Lecture 2 – Hardware (Fluidics, Optics and Electronics)

11:30-12:15 Lecture 3 – Compensation Principles and Best Practices

12:15-13:15 Lunch and Sponsor Talk

13:15-14:00 Activity 2 – Compensation practice (w/data)

14:00-14:45 Lecture 4 – Controls in Flow Cytometry

14:45-15:30 Lecture 5 – Understanding Flow Cytometry Data

15:30-15:45 Coffee Break and questions

15:30-16:30 Lecture 6 – Optimization and Standardization in Flow Cytometry

16:30-17:00 Activity 3 – Test yourself

Day 2 – Advanced Topics

09:00-09:45 Lecture 7 – Theory of Polychromatic Panel Design

09:45-10:30 Activity 4– Panel design

10:30-10:45 Coffee Break and Questions

10:45-11:45 Lecture 8 – Statistics in Flow Cytometry

12:00-13:00 Lunch and Sponsor Talk

13:00-13:45 Lecture 9: Advanced topic – Cell Cycle Analysis

13:45-14:30 Lecture 10: Advanced topic – Apoptosis

14:30-15:15 Activity 5 – data analysis

15:15-15:30 Coffee Break and questions

15:30-16:15 Lecture 11: Advanced topic – CyTOF (beyond 18 colors)

16:15-17:00 Lecture 12: Troubleshooting your data

Tim Bushnell, Ph.D.; Director of the URMC Shared Resource Labs

Organizer of MUSC Advanced Flow Cytometry Training

Tim is an expert flow cytometrist and technical applications scientist. His goal is to help researchers get the best possible data out of their flow cytometry experiments as possible. He is the director of one of the largest medical research cores in the world and is the co-founder of Expert Cytometry. Tim’s specialities include flow cytometry, cell sorting, design of polychromatic panels, data analysis, quality control, core management operation, training, and education. Here is a brief history of Tim’s appointments:

2012, Appointed as Director of the URMC Shared Resource Labs.
2011, Appointed Assistant Professor of Pediatrics and Hematology.
2008, Appointed Research Assistant Professor and Director of the URMC flow cytometry core.
2004, Founded the WNYFUG group, served on the board of GLIIFCA, currently serving on the Executive Committee of ISAC since 2008, currently the Secretary and co-chair of the Membership Services committee.
2003, Appointed the Director of the Center for Pediatric Biomedical Research’s Flow Cytometry Facility at the University of Rochester Medical Center.


March 28, 2017
March 29, 2017
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