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MUSC Advanced Flow Cytometry Training
March 28, 2017 - March 29, 2017
Educational Objectives – This two-day course will start by covering the fundamental areas critical for successful flow cytometry: Reagents, Hardware, Controls, Compensation, and Data Analysis. The second day will build upon these concepts with a focus on practical application of flow cytometry including: Polychromatic Panel Design, Statistics, Cell Cycle analysis, Apoptosis and Troubleshooting. At the end of the course, attendee’s will have firm understanding of the principles and how these apply to developing, executing, analyzing and troubleshooting flow cytometry experiments
Target Audience: Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color experiments). Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.
Day 1 – Principles of Flow Cytometry
09:00-09:30 Course Introduction and Activity 1 – Design the Experiment
09:30-10:15 Lecture 1 – Software (Reagents, Fluorescence and Fluorochromes)
10:15-10:30 Introduction to Spectral Viewers
10:30-10:45 Coffee Break and questions
10:45-11:30 Lecture 2 – Hardware (Fluidics, Optics and Electronics)
11:30-12:15 Lecture 3 – Compensation Principles and Best Practices
12:15-13:15 Lunch and Sponsor Talk
13:15-14:00 Activity 2 – Compensation practice (w/data)
14:00-14:45 Lecture 4 – Controls in Flow Cytometry
14:45-15:30 Lecture 5 – Understanding Flow Cytometry Data
15:30-15:45 Coffee Break and questions
15:30-16:30 Lecture 6 – Optimization and Standardization in Flow Cytometry
16:30-17:00 Activity 3 – Test yourself
Day 2 – Advanced Topics
09:00-09:45 Lecture 7 – Theory of Polychromatic Panel Design
09:45-10:30 Activity 4– Panel design
10:30-10:45 Coffee Break and Questions
10:45-11:45 Lecture 8 – Statistics in Flow Cytometry
12:00-13:00 Lunch and Sponsor Talk
13:00-13:45 Lecture 9: Advanced topic – Cell Cycle Analysis
13:45-14:30 Lecture 10: Advanced topic – Apoptosis
14:30-15:15 Activity 5 – data analysis
15:15-15:30 Coffee Break and questions
15:30-16:15 Lecture 11: Advanced topic – CyTOF (beyond 18 colors)
16:15-17:00 Lecture 12: Troubleshooting your data
Tim Bushnell, Ph.D.; Director of the URMC Shared Resource Labs
Organizer of MUSC Advanced Flow Cytometry Training
Tim is an expert flow cytometrist and technical applications scientist. His goal is to help researchers get the best possible data out of their flow cytometry experiments as possible. He is the director of one of the largest medical research cores in the world and is the co-founder of Expert Cytometry. Tim’s specialities include flow cytometry, cell sorting, design of polychromatic panels, data analysis, quality control, core management operation, training, and education. Here is a brief history of Tim’s appointments:
2012, Appointed as Director of the URMC Shared Resource Labs.
2011, Appointed Assistant Professor of Pediatrics and Hematology.
2008, Appointed Research Assistant Professor and Director of the URMC flow cytometry core.
2004, Founded the WNYFUG group, served on the board of GLIIFCA, currently serving on the Executive Committee of ISAC since 2008, currently the Secretary and co-chair of the Membership Services committee.
2003, Appointed the Director of the Center for Pediatric Biomedical Research’s Flow Cytometry Facility at the University of Rochester Medical Center.